Improving ultrasound gene transfection efficiency by controlling ultrasound excitation of microbubbles
نویسندگان
چکیده
منابع مشابه
Ultrasound-Mediated Gene Delivery with Cationic Versus Neutral Microbubbles: Effect of DNA and Microbubble Dose on In Vivo Transfection Efficiency
OBJECTIVE To assess the effect of varying microbubble (MB) and DNA doses on the overall and comparative efficiencies of ultrasound (US)-mediated gene delivery (UMGD) to murine hindlimb skeletal muscle using cationic versus neutral MBs. MATERIALS AND METHODS Cationic and control neutral MBs were characterized for size, charge, plasmid DNA binding, and ability to protect DNA against endonucleas...
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The importance of the excitation bandwidth is well known in diagnostic ultrasound imaging. However, the effect of excitation bandwidth in therapeutic applications of microbubbles has been mostly overlooked. A majority of contrast agent production techniques generate polydisperse microbubble populations, so a wide range of resonance frequencies exist. Therefore, wideband excitation is necessary ...
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Gene therapy and treatment with siRNA hold potential to treat a wide variety of different diseases. Genetic material is not usually stable and is generally hydrolyzed following intravascular administration. This makes the delivery of genes somewhat problematic since intact genetic material must usually be delivered intracellularly for therapeutic effect. For gene therapy, in most cases the gene...
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Gene therapy provides an efficient approach for treatment of cardiovascular disease. To realize the therapeutic effect, both efficient delivery to the target cells and sustained expression of transgenes are required. Ultrasound targeted microbubble destruction (UTMD) technique has become a potential strategy for target-specific gene and drug delivery. When gene-loaded microbubble is injected, t...
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Conventional ultrasound has well known short comings in the detection and characterisation of focal liver lesions. A false negative rate of up to 30% has been quoted in the detection of liver metastases and its accuracy in the characterisation of focal liver lesions is poor compared to computed tomography (CT) and magnetic resonance (MR). Ultrasound microbubble contrast agents have redressed th...
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ژورنال
عنوان ژورنال: Journal of Controlled Release
سال: 2013
ISSN: 0168-3659
DOI: 10.1016/j.jconrel.2013.05.039